NGF Recombinant Proteins
Nerve Growth Factor (NGF) is a member of the neurotrophin family, crucial for the survival, differentiation, and maintenance of neurons in the peripheral and central nervous systems. NGF recombinant proteins are widely used in neuroscience research, therapeutic development, and cellular studies to investigate mechanisms of neuroprotection, axonal growth, and neuronal plasticity.
Content
- Structure and Function:
- NGF is a dimeric protein with each monomer containing 118 amino acids.
- It binds to TrkA for pro-survival signaling and to p75NTR for additional signaling pathways influencing cell fate.
- Dysregulation of NGF is implicated in Alzheimer’s disease, chronic pain, and other neurological conditions.
- Expression Systems:
- Bacterial Systems (e.g., E. coli): Ideal for producing biologically active NGF at a lower cost.
- Mammalian Systems: Preferred for producing NGF with native post-translational modifications and proper folding.
- Yeast and Insect Systems: Provide a balance between scalability and protein fidelity.
Applications
- Neurodegenerative Disease Research:
- Investigating NGF's role in Alzheimer’s disease, focusing on cholinergic neuron survival.
- Exploring NGF-mediated neuroprotection and repair in Parkinson’s disease models.
- Drug Discovery and Therapeutic Development:
- High-throughput screening of small molecules targeting NGF or its receptors.
- Testing NGF analogs and delivery systems for treating neurological disorders.
- Cellular and Developmental Biology:
- Studying NGF's role in axonal growth, differentiation, and synaptic plasticity.
- Investigating interactions with TrkA and p75NTR in neuronal and non-neuronal cells.
NGF recombinant proteins are vital for advancing neuroscience research and therapeutic strategies targeting neurodegeneration and chronic pain. Their biological relevance and versatility position them as indispensable tools in basic and applied research.
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