NUDT Recombinant Proteins 

NUDT (Nudix Hydrolase) recombinant proteins belong to a family of enzymes characterized by their ability to hydrolyze nucleoside diphosphate derivatives. These proteins are vital in maintaining cellular nucleotide pool balance by degrading potentially harmful nucleotide metabolites, including oxidized forms like 8-oxo-dGTP. NUDT enzymes safeguard genomic stability and are implicated in various cellular processes, including signal transduction and oxidative stress response.

Content of NUDT Recombinant Protein

NUDT recombinant proteins are produced using advanced expression systems (e.g., E. coli or mammalian cells) to ensure their proper folding and activity. These proteins are typically tagged (e.g., His-tag, GST-tag) for streamlined purification and detection. Their design includes highly conserved Nudix motifs required for catalytic activity. Recombinant NUDT proteins, such as NUDT1 (MTH1) and NUDT5, are widely studied for their roles in oxidative damage repair and nucleotide metabolism.

Applications of NUDT Recombinant Protein

• Oxidative Damage Repair
  Used in studies focusing on the degradation of oxidized nucleotides, preventing incorporation into nucleic acids.
• Cancer Research
  Essential for screening small-molecule inhibitors targeting NUDT1 (MTH1), a key enzyme in tumor cell survival under oxidative stress.
• Drug Discovery
  Serve as targets for the development of novel therapeutics against oxidative stress-related diseases.
• Structural and Functional Studies
  Employed in crystallographic studies to elucidate the enzyme’s active site architecture and mechanism.
• Diagnostic Assay Development
  Utilized in designing enzymatic assays for monitoring NUDT activity as a biomarker in neurodegenerative and metabolic disorders.
• Synthetic Biology
  Incorporated into engineered pathways to regulate nucleotide degradation and enhance metabolic stability.

NUDT recombinant proteins are indispensable tools in molecular biology, enzymology, and therapeutic research, offering insights into nucleotide metabolism and oxidative stress management.