OROV Recombinant Proteins 

OROV recombinant proteins are synthetic proteins engineered to mimic the structure and function of proteins from the Oropouche virus (OROV). OROV is an arbovirus belonging to the Orthobunyavirus genus within the family Peribunyaviridae, known for causing febrile illnesses and neurological complications in humans. Recombinant protein technology enables the production of highly purified OROV proteins for research, diagnostics, and therapeutic applications. These proteins are typically expressed in systems such as E. coli, mammalian cells, or insect cells to achieve the required structural fidelity and post-translational modifications.

Technical Content

• Structure and Features:
  • Common OROV recombinant proteins include the Nucleocapsid (N), Glycoproteins (Gn/Gc), and Non-Structural Proteins (NSs).
  • These proteins are produced with high purity using advanced chromatographic techniques.
  • Tags such as His-tag or GST-tag may be included for enhanced purification and detection.
• Expression Systems:
  • Bacterial Systems (E. coli): Efficient for non-glycosylated proteins like the Nucleocapsid protein.
  • Mammalian Systems: Suitable for glycosylated proteins like Gn/Gc to maintain biological activity.
  • Insect Cell Systems: Frequently used for complex OROV protein structures requiring post-translational modifications.
• Quality Control:
  • Validated using SDS-PAGE, Western Blot, and ELISA.
  • Tested for purity (>90%) and functional activity in downstream applications.
  • Endotoxin levels are minimized (<1 EU/µg) for sensitive applications.

Applications of OROV Recombinant Proteins

• Diagnostic Assays:
  • Used as antigens in ELISA and Immunoblot to detect OROV-specific antibodies.
  • Critical in developing diagnostic kits for serological surveillance in endemic regions.
• Vaccine Development:
  • Serve as immunogens to evaluate vaccine candidates for OROV.
  • Aid in testing neutralizing antibody responses.
• Therapeutic Research:
  • Enable structural and functional studies to identify therapeutic targets for antiviral drug development.
  • Facilitate the screening of inhibitors that block viral entry or replication.
• Basic Virology Research:
  • Assist in understanding the molecular biology of OROV replication and pathogenesis.
  • Employed in protein-protein interaction studies to characterize host-virus interactions.

OROV recombinant proteins provide indispensable tools for advancing research in virology, immunology, and therapeutic development. Their precise production and validation ensure reliable performance in diagnostics and experimental applications, making them a cornerstone in the study of Oropouche virus and related orthobunyaviruses.