PRDX Recombinant Proteins 

PRDX (Peroxiredoxin) recombinant proteins are engineered antioxidant enzymes belonging to the peroxiredoxin family. These proteins play a crucial role in cellular redox regulation by reducing hydrogen peroxide (H₂O₂), organic hydroperoxides, and peroxynitrite, thereby protecting cells from oxidative stress. PRDX recombinant proteins are essential for studying redox biology, cellular signaling, and the role of oxidative stress in disease.

Content on PRDX Recombinant Proteins

• Structure and Function
  • PRDX proteins are thiol-specific peroxidases that utilize cysteine residues in their active site to catalyze the reduction of peroxides.
  • They are classified into three types based on their catalytic mechanisms:
    • Typical 2-Cys PRDXs (e.g., PRDX1, PRDX2): Form intersubunit disulfide bonds.
    • Atypical 2-Cys PRDXs (e.g., PRDX5): Form intrasubunit disulfide bonds.
    • 1-Cys PRDXs (e.g., PRDX6): Utilize a single cysteine residue for catalysis.
• Expression Systems
  • Prokaryotic Systems (e.g., E. coli): Ideal for producing large quantities of recombinant PRDX with active peroxidase domains.
  • Eukaryotic Systems (e.g., yeast, insect, or mammalian cells): Preserve post-translational modifications necessary for specific cellular applications.

Applications of PRDX Recombinant Proteins

• Oxidative Stress Research
  • Study the role of PRDX proteins in detoxifying reactive oxygen species (ROS) and maintaining redox homeostasis.
  • Investigate their contribution to cellular defense mechanisms against oxidative damage.
• Disease Research
  • Cancer Biology: Investigate the dual role of PRDXs in promoting tumor survival under oxidative stress and their potential as therapeutic targets.
  • Neurodegenerative Diseases: Study PRDX dysfunction in diseases like Parkinson’s and Alzheimer’s, where oxidative stress plays a central role.
  • Cardiovascular Diseases: Assess PRDX activity in ischemia-reperfusion injury and atherosclerosis.
• Drug Discovery and Development
  • Inhibitor Screening: Identify molecules targeting PRDX activity for therapeutic modulation.
  • Therapeutic Design: Use PRDXs as antioxidant agents in models of oxidative stress-induced diseases.

PRDX recombinant proteins are indispensable tools in redox biology and oxidative stress research, offering valuable insights into the molecular mechanisms underlying health and disease.