Proteasome Recombinant Proteins 

Proteasome recombinant proteins represent critical tools for studying proteolytic pathways and cellular protein homeostasis. The proteasome is a multicatalytic protease complex responsible for degrading ubiquitinated proteins, regulating key cellular processes such as cell cycle, apoptosis, and immune responses. Recombinant proteasome proteins, including core subunits (20S) and regulatory particles (19S), are produced to investigate proteasome activity, assembly, and interactions in various biological contexts.

Content

Proteasome recombinant proteins are synthesized using expression systems that support proper folding and activity, such as bacterial, yeast, or mammalian systems. These recombinant proteins can include full proteasome complexes, specific subunits, or mutant forms designed to study catalytic activity, structural assembly, or interactions with inhibitors.

Applications

• Basic Proteostasis Research:
  • Enables studies on protein degradation mechanisms.
  • Used to dissect the role of proteasome subunits in cellular signaling and stress responses.
• Assay Development:
  • Utilized in the development of proteasome activity assays for research or diagnostic purposes.
  • Facilitates biomarker discovery in diseases linked to proteasome dysregulation.
• Structural Biology:
  • Aids in elucidating the 3D architecture and dynamics of proteasome assembly.
  • Supports mutagenesis studies to identify critical residues involved in catalytic activity or interactions.
• Immune System Research:
  • Investigating immunoproteasome function, which is crucial for antigen processing and presentation via MHC class I molecules.
  • Analyzing the role of proteasome in immune cell signaling and regulation.

The versatility and high-quality production of proteasome recombinant proteins make them indispensable in modern molecular biology and therapeutic research.