PSMB Recombinant Proteins
PSMB (Proteasome Subunit Beta) recombinant proteins are engineered components of the proteasome complex, specifically the beta subunits involved in proteolytic activity. The proteasome is a multicatalytic proteinase complex critical for degrading ubiquitinated proteins, regulating protein homeostasis, and controlling numerous cellular processes, including cell cycle, immune responses, and apoptosis.
Content on PSMB Recombinant Proteins
- Structure and Function
- PSMB proteins are beta subunits of the 20S proteasome core particle, with catalytic activity confined to specific subunits (e.g., PSMB5, PSMB6, and PSMB7).
- The proteasome functions as a proteolytic machine, degrading proteins into peptides for antigen presentation, turnover, and cellular regulation.
- Immunoproteasome variants (e.g., PSMB8, PSMB9, and PSMB10) are specialized for antigen processing in the immune system.
- Expression Systems
- Prokaryotic Systems (e.g., E. coli): Suitable for producing recombinant PSMB subunits for enzymatic and structural studies.
- Eukaryotic Systems (e.g., mammalian or insect cells): Preserve post-translational modifications necessary for native-like folding and activity.
Applications of PSMB Recombinant Proteins
- Drug Discovery and Development
- Screen inhibitors targeting PSMB5 or other catalytic subunits for cancer therapy, such as proteasome inhibitors like bortezomib.
- Develop immunoproteasome-specific inhibitors for treating autoimmune diseases.
- Cancer Research
- Study the role of PSMB proteins in proteasomal degradation of regulatory proteins involved in cell cycle and apoptosis.
- Investigate the impact of proteasome dysfunction on tumor progression and drug resistance.
- Neurodegenerative Disease Studies
- Examine proteasome activity in neurodegenerative diseases like Alzheimer’s and Parkinson’s, where protein aggregation is a hallmark.
- Use recombinant PSMB proteins to analyze the degradation of misfolded proteins.
PSMB recombinant proteins are essential for understanding proteasome biology and its implications in immunology, oncology, and neurodegeneration, offering valuable insights for therapeutic development.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|