RBM Recombinant Proteins 

RBM recombinant proteins refer to synthetically produced versions of RNA-binding motif (RBM) proteins, which are a family of proteins involved in RNA processing, splicing, stability, and translation. RBM proteins contain one or more RNA recognition motifs (RRMs) that facilitate their interaction with RNA molecules, playing critical roles in post-transcriptional gene regulation. These proteins are essential for studying RNA biology, cellular stress responses, and disease mechanisms, particularly in cancer and neurological disorders.

Technical Content

• Expression and Features:
  • Typically produced in E. coli, baculovirus-infected insect cells, or mammalian systems, depending on post-translational modification requirements.
  • Purified recombinant RBM proteins often include His-tag or GST-tag for ease of purification and detection.
  • Available as full-length proteins or specific domains (e.g., RNA-binding motifs) for targeted studies.
• Quality and Validation:
  • Purity: Verified to exceed 90–95% using SDS-PAGE and chromatographic techniques.
  • Functional Assays: Tested for RNA-binding activity using EMSA (Electrophoretic Mobility Shift Assay) or CLIP-seq (Cross-linking and Immunoprecipitation Sequencing).
  • Structural Validation: Confirmed via mass spectrometry and circular dichroism for proper folding.
• Stability and Solubility:
  • Optimized buffer conditions ensure stability and solubility for structural studies and biochemical assays.

Applications of RBM Recombinant Proteins

• Cancer Research:
  • RBM5 and RBM10 are tumor suppressors that regulate apoptosis and cell cycle through alternative splicing.
  • Recombinant RBM proteins are used in functional assays to explore their role in cancers such as lung cancer and breast cancer.
• Neurological Disorders:
  • RBM3 is studied for its neuroprotective roles in conditions such as hypothermia-induced ischemia and Alzheimer’s disease.
  • Functional assays with recombinant RBM3 help identify therapeutic targets for neurodegeneration.
• Structural Biology:
  • Recombinant RBM proteins are used in crystallography and NMR studies to elucidate the structure of RNA-protein complexes.
  • Provide insights into the mechanisms of RNA recognition and binding specificity.

RBM recombinant proteins are indispensable tools for advancing the understanding of RNA processing, gene regulation, and their implications in diseases. Their applications span RNA biology, oncology, neurobiology, and therapeutic discovery, making them essential for addressing fundamental and translational research challenges.