RFC Recombinant Proteins
RFC (Replication Factor C) recombinant protein is a critical component of the DNA replication machinery, functioning as a DNA-dependent ATPase. RFC plays a key role in loading and unloading the proliferating cell nuclear antigen (PCNA) clamp onto DNA during replication and repair. RFC is essential for maintaining genomic stability, facilitating replication fork progression, and coordinating DNA damage repair pathways.
Content of RFC Recombinant Protein
RFC recombinant proteins are produced in heterologous systems such as bacterial, insect, or mammalian cells to ensure their functional integrity. These proteins often include the conserved subunits of the RFC complex, particularly the RFC1 subunit, which is crucial for DNA binding and PCNA interaction. Recombinant RFC may include affinity tags (e.g., His-tag or GST-tag) to facilitate purification and experimental applications.
Applications of RFC Recombinant Protein
- DNA Replication Studies
- Investigate the role of RFC in loading PCNA and coordinating replication fork stability.
- DNA Repair Mechanisms
- Study RFC's involvement in post-replicative DNA repair processes, such as mismatch repair and nucleotide excision repair.
- Cancer Research
- RFC dysfunction is implicated in tumorigenesis. Recombinant RFC is used to study its role in genomic instability and cancer progression.
- Drug Discovery
- High-throughput screening assays use RFC recombinant protein to identify inhibitors targeting replication machinery, with implications for cancer therapy.
- Protein-Protein Interaction Studies
- Explore interactions between RFC, PCNA, DNA polymerases, and other replication-associated proteins.
- Structural Biology
- Used in crystallography and spectroscopic studies to elucidate the structural basis of clamp loading and ATPase activity.
RFC recombinant proteins are indispensable for understanding the molecular mechanisms of DNA replication and repair, making them vital tools in cancer biology, genomic stability research, and therapeutic development.
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