RGP Recombinant Proteins 

RGP (Rhamnogalacturonan I Lyase) recombinant protein is an enzyme that specifically cleaves rhamnogalacturonan I (RG-I), a complex pectic polysaccharide found in plant cell walls. RGP enzymes are crucial for studying plant cell wall degradation, biomass conversion, and plant-pathogen interactions. In addition, RGP proteins have applications in biotechnology for biofuel production and functional carbohydrate research.

Content of RGP Recombinant Protein

RGP recombinant proteins are expressed in bacterial, yeast, or other expression systems to maintain enzymatic activity and structural integrity. They are typically engineered with affinity tags (e.g., His-tag or GST-tag) for purification and detection. The proteins retain conserved catalytic domains required for their lyase activity, enabling them to cleave the RG-I backbone via a β-elimination mechanism.

Applications of RGP Recombinant Protein

• Plant Cell Wall Research
  Used to investigate the structure and composition of plant cell walls, particularly the role of RG-I in wall integrity and remodeling.
• Biomass Conversion
  RGP enzymes are critical for breaking down plant biomass into fermentable sugars, supporting biofuel production research.
• Enzymatic Assays
  Employed in assays to study substrate specificity, kinetic properties, and interaction with other plant cell wall-degrading enzymes.
• Plant Pathology
  RGP proteins are studied to understand their role in microbial pathogenesis and plant defense mechanisms.
• Carbohydrate Research
  Used to produce and analyze functional oligosaccharides derived from RG-I, which have potential applications in food, health, and cosmetics.
• Biotechnological Applications
  Recombinant RGP enzymes are utilized in the enzymatic processing of plant-derived materials for industrial applications.

RGP recombinant protein is an essential tool in plant science, biotechnology, and industrial applications, enabling advancements in both basic and applied research.