SDC Recombinant Proteins 

SDC recombinant proteins refer to synthetic versions of Syndecans (SDCs), a family of transmembrane heparan sulfate proteoglycans involved in cell-cell and cell-matrix interactions. Syndecans play pivotal roles in regulating cell signaling pathways, cytoskeletal organization, and extracellular matrix remodeling. SDC recombinant proteins, particularly SDC1, SDC2, SDC3, and SDC4, are widely utilized in research on cancer, angiogenesis, wound healing, and tissue regeneration due to their involvement in processes like cell adhesion, proliferation, and migration.

Technical Content

• Production and Features:
  • Recombinant SDC proteins are typically produced in mammalian cell systems to ensure correct post-translational modifications such as glycosylation and sulfation.
  • E. coli systems may be used for truncated or core protein forms lacking post-translational modifications.
  • Commonly tagged with His-tag or Fc-tag for simplified purification and detection.
• Validation and Quality Control:
  • Purity: Achieved to levels >90% through affinity chromatography.
  • Characterization: Confirmed by SDS-PAGE, Western blotting, and mass spectrometry.
  • Functional Testing: Verified in binding assays with heparin or growth factors to assess activity.
• Solubility and Stability:
  • Recombinant SDC proteins are optimized for stability in aqueous solutions.
  • Retain their functional domains, including extracellular heparan sulfate chains and protein cores, for biological activity.

Applications of SDC Recombinant Proteins

• Cancer Research:
  • SDC1 is a biomarker for tumor progression and metastasis, particularly in breast, lung, and multiple myeloma cancers.
  • Recombinant SDC1 is used in functional assays to study interactions with growth factors like FGF and VEGF, which promote angiogenesis and tumor proliferation.
  • Evaluating therapeutic strategies targeting SDC1 for cancer inhibition.
• Neuroscience:
  • SDC3 facilitates neurogenesis and axonal guidance, making it a focus for understanding neural plasticity and injury recovery.
  • Recombinant SDC3 is used in assays exploring its interaction with brain-derived neurotrophic factors (BDNFs) and other signaling molecules.
• Wound Healing and Tissue Regeneration:
  • SDC4 regulates integrin-mediated cell adhesion and cytoskeletal remodeling, critical for wound closure and tissue repair.
  • Applications in developing therapeutic approaches for chronic wounds and regenerative medicine.
• Developmental Biology:
  • Syndecans, particularly SDC2, are studied for their role in embryogenesis, morphogenesis, and tissue patterning.
  • Recombinant proteins help elucidate their interaction with matrix proteins like fibronectin and laminin.

SDC recombinant proteins are critical tools for investigating the diverse roles of Syndecans in cell signaling, matrix remodeling, and tissue repair. Their precise production and functional validation enable researchers to delve deeper into cellular mechanisms and develop targeted therapeutic strategies for cancer, neural disorders, and regenerative medicine.