TNF Recombinant Proteins 

Tumor Necrosis Factor (TNF) is a cytokine primarily involved in inflammation, immune regulation, and apoptosis. It is a critical signaling molecule in the tumor necrosis factor superfamily and plays a central role in immune responses, cell death, and survival pathways. Recombinant TNF proteins are synthetically produced, enabling researchers to study its biological effects under controlled conditions.

Content of TNF Recombinant Protein

• Structure:
  • Composed of the biologically active soluble form or full-length transmembrane protein.
  • Typically derived from human, mouse, or other species-specific sequences for species-specific studies.
  • Expressed in systems like E. coli, HEK293, or CHO cells, with mammalian expression systems producing post-translationally modified proteins.
• Physical and Biochemical Properties:
  • Molecular Weight: Ranges from ~17 kDa (monomer) to ~51 kDa (homotrimer).
  • Stability: Supplied in lyophilized or liquid form with stabilizing agents.
  • Solubility: Highly soluble in physiological buffers.
  • Endotoxin Level: Less than 1 EU/µg to minimize interference in biological assays.

Applications of TNF Recombinant Protein

• Cancer Research:
  • Used to study TNF-mediated apoptosis and necroptosis in tumor cells.
  • Helps in understanding the dual role of TNF in tumor suppression and tumor progression.
• Drug Discovery and Development:
  • Screening of TNF inhibitors (e.g., monoclonal antibodies or small molecules) for therapeutic use in inflammatory diseases and cancer.
  • Evaluates drug candidates for their ability to modulate TNF signaling pathways.
• Cell Biology:
  • Examines the signaling pathways downstream of TNFR1 and TNFR2 receptors, such as NF-κB, JNK, and MAPK activation.
  • Studies the balance between TNF-mediated cell survival and cell death in various biological contexts.

Recombinant TNF proteins provide a reliable and precise tool for investigating the multifaceted role of TNF in immune responses, cell death, and inflammatory diseases. Their availability in high purity and functional activity ensures accurate and reproducible data across various experimental platforms.