TREM Recombinant Proteins 

Triggering Receptor Expressed on Myeloid Cells (TREM) proteins are critical immunoregulatory receptors primarily expressed on myeloid lineage cells, including macrophages, neutrophils, and microglia. TREM family members, such as TREM1 and TREM2, are involved in inflammation, phagocytosis, and immune response modulation. Dysregulation of TREM proteins is associated with diseases like Alzheimer’s disease, sepsis, and autoimmune disorders.

Content of TREM Recombinant Proteins

• Production:
  • Expressed in mammalian or insect cell systems to ensure proper folding and glycosylation.
  • Purified using affinity chromatography methods, such as His-tag or Fc-tag purification.
• Variants:
  • Full-length recombinant TREM proteins for studying receptor functionality.
  • Extracellular domains for ligand-binding assays and antibody generation.
  • Mutant forms to investigate structural and functional aspects of disease-associated variants.
• Specifications:
  • Purity: >90% as confirmed by SDS-PAGE and HPLC.
  • Activity: Validated through functional assays such as ligand binding or receptor activation.
  • Stability: Supplied in buffered solutions (e.g., PBS or HEPES) with cryoprotectants for long-term storage.

Applications of TREM Recombinant Proteins

• Neurodegenerative Disease Research:
  • TREM2 recombinant proteins are used to study microglial function in Alzheimer’s disease, including their role in amyloid-beta uptake and clearance.
  • Investigation of mutations like TREM2 R47H, associated with increased Alzheimer’s disease risk.
• Inflammation and Immune Response Studies:
  • TREM1 recombinant proteins are applied to research its role in amplifying inflammatory responses in sepsis and autoimmune diseases.
  • Analyze interactions with downstream signaling molecules such as DAP12.
• Drug Discovery and Screening:
  • High-throughput screening of inhibitors or agonists targeting TREM1 and TREM2 for therapeutic development.
  • Evaluation of monoclonal antibodies or small molecules that modulate TREM signaling pathways.

TREM recombinant proteins are vital tools for studying the immunoregulatory roles of TREM receptors in health and disease. Their applications span neurodegeneration, inflammation, and therapeutic development, making them indispensable in immunology and biomedical research.