UBTF Recombinant Proteins 

UBTF (Upstream Binding Transcription Factor) recombinant proteins are derived from the UBTF gene, which encodes a critical transcription factor involved in the regulation of ribosomal RNA (rRNA) synthesis. UBTF is a chromatin-binding protein that interacts with rDNA promoters, modulating the transcriptional activity of RNA polymerase I. Its role in ribosome biogenesis makes UBTF essential for cell growth, proliferation, and response to cellular stress.

Content of UBTF Recombinant Proteins

UBTF recombinant proteins are produced in heterologous expression systems such as bacterial, insect, or mammalian cells. These proteins retain conserved domains, including the high-mobility group (HMG) box domains, which are required for DNA binding and chromatin remodeling. They are typically provided with purification tags (e.g., His-tag, GST-tag) to facilitate isolation and downstream experimental use.

Applications of UBTF Recombinant Proteins

• Ribosomal Biogenesis Research
  • Investigate the role of UBTF in rRNA transcription and ribosome assembly.
  • Explore its regulation by upstream signaling pathways, such as mTOR and AMPK.
• Cancer Studies
  • UBTF is upregulated in several cancers, making it a target for understanding tumor growth and proliferation.
  • Used to study its interaction with oncogenic pathways that drive cellular metabolism.
• Chromatin and Epigenetic Research
  • UBTF recombinant proteins are used to examine chromatin remodeling and its impact on rDNA promoter accessibility.
• Transcriptional Regulation
  • Employed in studies of RNA polymerase I machinery to uncover the mechanisms of rRNA synthesis under varying physiological conditions.
• Drug Development
  • Screen small molecules or inhibitors targeting UBTF activity, especially in diseases where dysregulated ribosome biogenesis is implicated.
• Stress Response Studies
  • Explore UBTF's role in cellular responses to environmental or metabolic stress, including nutrient deprivation and oxidative stress.

UBTF recombinant proteins are indispensable tools for advancing research in transcriptional regulation, cancer biology, and cellular metabolism, offering insights into the fundamental processes of ribosome biogenesis and gene expression.