USP Recombinant Proteins 

USP (Ubiquitin-Specific Protease) recombinant proteins are a class of deubiquitinating enzymes (DUBs) that remove ubiquitin or ubiquitin-like modifiers from target proteins. This activity is critical for maintaining protein homeostasis, regulating signal transduction, and controlling cellular processes such as DNA repair, cell cycle progression, and apoptosis. USPs play a significant role in various physiological and pathological conditions, including cancer, neurodegenerative disorders, and infectious diseases.

Content of USP Recombinant Protein

USP recombinant proteins are produced in heterologous expression systems (e.g., bacterial, insect, or mammalian cells) to ensure proper folding and activity. These proteins are often tagged (e.g., His-tag or GST-tag) for simplified purification and downstream applications. Specific USPs, such as USP7, USP14, and USP28, are widely studied due to their roles in diverse biological pathways. Recombinant USPs retain their catalytic activity and are designed to preserve functional domains necessary for substrate recognition and interaction.

Applications of USP Recombinant Protein

• Proteostasis Research
  Used to study the regulation of ubiquitin-dependent protein degradation and stabilization, particularly in the ubiquitin-proteasome system.
• Cancer Biology
  USP recombinant proteins, such as USP7, are employed in studies exploring their role in tumorigenesis, oncogenic signaling, and drug resistance.
• Drug Discovery
  Critical for screening small-molecule inhibitors targeting DUBs, facilitating therapeutic development for cancers, neurodegeneration, and viral infections.
• DNA Damage and Repair Studies
  USPs involved in DNA repair pathways, such as USP1, are investigated for their roles in maintaining genomic stability.
• Neurobiology
  USPs like USP14 and USP30 are studied for their impact on neurodegenerative diseases, including Parkinson’s and Alzheimer’s disease.
• Signal Transduction
  USP recombinant proteins are utilized to understand their regulatory role in signaling cascades, including NF-κB and WNT pathways.

USP recombinant proteins are indispensable for advancing the understanding of ubiquitin biology, offering insights into therapeutic strategies for managing diseases driven by dysregulated ubiquitination.