DNA Recombinant Proteins
DNA recombinant proteins are engineered proteins produced using recombinant DNA technology, where specific DNA sequences coding for proteins are inserted into expression vectors and expressed in host cells. These proteins are often used to study DNA-protein interactions, DNA repair mechanisms, replication, transcription, and other essential genomic processes.
Production and Expression
- Produced in prokaryotic (e.g., E. coli) or eukaryotic systems (e.g., insect or mammalian cells) depending on post-translational modification requirements.
- Optimized for high yield and functional integrity.
- Batch consistency for reproducible results in experimental setups.
Applications of DNA Recombinant Proteins
- Molecular Biology Research
- Investigate protein-DNA interactions crucial in gene regulation, chromatin remodeling, and epigenetics.
- Study DNA repair pathways, including homologous recombination, non-homologous end joining, and base excision repair.
- Cancer Research
- Evaluate the role of DNA-binding proteins (e.g., p53, BRCA1) in genome stability and tumor suppression.
- Develop inhibitors targeting oncogenic DNA-binding proteins.
- Therapeutic Development
- Use in gene editing tools, such as CRISPR-associated proteins fused with DNA-binding modules.
- Explore potential therapeutic applications in targeting specific DNA sequences in genetic disorders.
- Structural and Functional Studies
- Determine the crystal structures of DNA-protein complexes to elucidate binding specificity and catalytic mechanisms.
- Analyze the role of conformational changes in protein activity.
- Industrial Biotechnology
- Used in synthetic biology for DNA replication and transcription systems.
- Key components in diagnostic assays and biosensor development.
DNA recombinant proteins are foundational tools in modern molecular biology and biotechnology, providing insights into the complex interactions and mechanisms governing DNA dynamics and enabling the development of innovative diagnostics and therapeutics.
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